![]() ![]() Convert images to grayscale or split channels if you want to detect cells in a different channel. Multichannel and RGB Images: The first channel, or the red color, will be what Cell Magic Wand sees. (1) Original image, (2) Median-filtered image, (3) ROI drawn on median filtered image, (4) ROI on original image. Miniature vibrators like the Topco Climax Minis Mighty Max Wand Vibe are also not compatible with the attachments offered here at Simpli Pleasure. Median Filtering: If there is significant contrast variability inside of your cells, try using a median filter to blur out those elements first. Keep in mind that some massagers and magic wands have a different head shape, such as the Liberte Intimate G-Spot Massager Vibe and The Almighty Hot-Cold Massager, and the attachments may not fit. From the ROI manager window, choose More->Options->Associate "Show All" ROIs with slices. Hide ROIs from other slices: If you're working with an image stack, you may want to make it so that ROIs only show up on the slice they came from. This will help you determine good values for Minimum and Maximum Diameter. If your units aren't in pixels, you must change the units to pixels first: do Analyze->Set Scale->Click to remove scale, and click OK. Length will be shown in the ImageJ main window, or you can press Ctrl M to bring up a measurement. Measure cell diameter: Use the Line ROI tool to draw a line across your cell. Shift-clicking on a cell you've already selected will deselect it. The ROI manager will appear and keep track of your selections. Shift-clicking: Hold down SHIFT to select multiple cells. A value of 0 will give you a circle - the smoothest ROI of all. ![]() ![]() Raise it for a rough ROI that delves into every nook and cranny on the outside of your cell. Lower it for a smooth ROI that follows the general shape of your cell. Roughness: This is a shape parameter that controls how rough the edge of your cell ROI will be. And having a smaller range of diameters to search through makes Cell Magic Wand run faster, too. Setting the minimum diameter higher helps Cell Magic Wand to ignore high-contrast elements inside your cell, such as nucleoli. In particular, setting the maximum diameter lower can prevent Cell Magic Wand from encircling multiple cells at once. But it'll work better the more information you give it. If you like, you can set the minimum diameter to 1 and the maximum diameter to something huge (like 1000). Maximum Diameter: What's the largest your cell could be (in pixels)? Minimum Diameter: What's the smallest your cell could be (in pixels)? Image Type: Are your cells brighter or darker than the background color? Parametersĭouble click on the tool to bring up this box. Restart ImageJ and select Cell Magic Wand Tool from the ">" menu on the right side of the toolbar:Ĭell Magic Wand will appear in your ImageJ toolbar: You may need to create the "Tools" directory.Įxample: C:\Program Files\ImageJ\plugins\Tools\Cell_Magic_Wand_Tool.jar. Install: Put Cell_Magic_Wand_Tool.jar into the plugins\Tools directory of your ImageJ installation. Version Required: ImageJ version 1.46d (January 2012) or later. ImageJ plugin for rapid human-assisted segmentation of cells in images. ![]()
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